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等離子熒光增強芯片檢測登革熱和塞卡病毒
Release date:2017-05-10 Already396 Read
 

We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections

斯坦福大學戴宏杰團隊在nature medicine上發文報道了在等離子體金平臺上同時檢測登革熱和塞卡病毒感染者的IgG、IgA抗體以及IgG抗體的親和力。他們發現,盡管這兩種病毒的IgM抗體檢測存在交叉反應,但是通過檢測塞卡病毒非結構蛋白1(NS1)抗原的IgG和IgA抗體,可特異性地檢測塞卡病毒感染。同時,他們發現,通過對IgG親和力的檢測可區分在登革熱流行區的患者近期ZIKV感染和過去DENV-2的感染。這種方法可以特意性地區分塞卡病毒與其它黃病毒屬類的感染

Bo Zhang et al. Diagnosis of Zika virus infection on a nanotechnology platform. Nature Medicine (2017) doi:10.1038/nm.4302

 
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